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Vol. 15. Issue 6.
Pages 528-532 (November - December 2011)
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Vol. 15. Issue 6.
Pages 528-532 (November - December 2011)
Original article
Open Access
Evaluation of the presence of microorganisms in solid-organ preservation solution
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André Marcelo Colvara Mattana1, Alexandre Rodrigues Marra2, Antônia Maria de Oliveira Machado3, Gaspar de Jesus Lopes Filho4, Alcides Augusto Salzedas Netto5, Adriano Miziara Gonzalez6,
Corresponding author
amgonzalez@uol.com.br

Correspondence to: Rua Napoleão de Barros, 610 Vila Clementino, 04024-002, São Paulo, SP Brazil, Phone: +55 11 5084-7551/5084-9131.
1 Postgraduate Student, Surgical Gastroenterology Service, Universidade Federal de São Paulo – Escola Paulista de Medicina (UNIFESP-EPM); General and Digestive Surgeon, Hospital Santo Antônio and Hospital Santa Catarina, Blumenal, SC, Brazil
2 Infectologist and Intensivist, Hospital Israelita Albert Einstein, SP, Brazil
3 Technical Director, Instituto Paulista de Doenças Infecciosas e Parasitárias; Technical Director, Laboratório Central do Hospital São Paulo, SP, Brazil
4 UNIFESP; Chief of Surgical Gastroenterology, Universidade Estadual Paulista (UNESP), SP, Brazil
5 Surgeon, Kidney Transplantation Program, Hospital do Câncer and Hospital Sírio-Libanês; Assistant of Pediatric Surgery, UNIFESP-EPM, SP, Brazil
6 Digestive Transplantation Coordinator, UNIFESP, SP, Brazil
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Abstract
Objective

To assess the presence of microorganism contamination in the preservation solution for transplant organs (kidney/pancreas).

Method

Between August 2007 and March 2008, 136 samples of preservation solution were studied prior to graft implantation. Variables related to the donor and to the presence of microorganisms in the preservation solution of organs were evaluated, after which the contamination was evaluated in relation to the “recipient culture” variable. Univariate and multivariate statistical analyses were performed.

Results

The contamination rate of the preservation solution was 27.9%. Coagulase-negative Staphylococcus was the most frequently isolated microorganism. However, highly virulent agents, such as fungi and enterobacteria, were also isolated. In univariate analysis, the variable “donor antibiotic use” was significantly associated to the contamination of the preservation solution. On the other hand, multivariate analysis found statistical significance in “donor antibiotic use” and “donor's infectious complications” variables.

Conclusions

In this study, 27.9% of the preservation solutions of transplant organs were contaminated. Infectious diseases and non-use of antibiotics by the donor were significantly related to the presence of microorganisms in organ preservation solutions. Contamination in organ preservation solutions was not associated with infection in the recipient.

Keywords:
organ transplantation
disease transmission, infectious
organ preservation solutions
microbial viability
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Copyright © 2011. Elsevier Editora Ltda.. All rights reserved
The Brazilian Journal of Infectious Diseases
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