TY - JOUR T1 - Association between the p53 arginine/arginine homozygous genotype at codon 72 and human papillomavirus E6/E7 mRNA expression JO - The Brazilian Journal of Infectious Diseases T2 - AU - Chuery,Ana Carolina Silva AU - Silva,Ismael Dale Cotrim Guerreiro da AU - Ribalta,Julisa Chamorro Lascasas AU - Speck,Neila Maria de Góis SN - 14138670 M3 - 10.1016/j.bjid.2017.03.002 DO - 10.1016/j.bjid.2017.03.002 UR - https://www.bjid.org.br/en-association-between-p53-arginine-arginine-homozygous-articulo-S1413867017302726 AB - ObjectiveTo evaluate the association between p53 polymorphisms and human papillomavirus (HPV) E6/E7 mRNA expression. MethodsWe analyzed 175 cervical samples from women aged 16–69 years old who were tested for HPV E6/E7 mRNA expression (NucliSENS® EasyQ® HPV). The samples were divided into three groups: positive (n=75) those with positive HPV E6/E7 mRNA expression and positive high-risk HPV Hybrid Capture (HR-HC) test; negative (n=52) those with negative HPV E6/E7 mRNA expression and positive HR-HC; and control (n=48) those with negative HPV E6/E7 mRNA expression and negative HR-HC. The p53 polymorphisms at codons 11, 72, and 248 were evaluated through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). ResultsThe frequency of the arginine/arginine homozygous genotype at codon 72 was significantly higher in the positive (49.3%) than in the negative (32.7%) and control groups (20.8%, p=0.002*). The frequency of the arginine allele was also significantly higher in the positive (67.3%) than in the negative (53.8%) and control groups (38.5%, p<0.001*). The arginine/arginine homozygous genotype was significantly associated with positive HPV E6/E7 mRNA expression (positive group) compared with negative and control groups (odds ratio: 2.633; 95% CI, 1.399–4.954, p=0.003). The frequency of arginine/arginine homozygous genotype at codon 72 remained significantly more frequent in the positive group of women aged ≥30 years than in the other two groups. ConclusionThe presence of the p53 arginine/arginine homozygous genotype at codon 72 was significantly associated with the positive HPV E6/E7 mRNA expression. ER -