Molecular characterization of viruses associated with gastrointestinal infection in HIV-positive patients

Silva, Raquel C.; Benati, Fabrício J.; Pena, Giselle P.A.; Santos, Norma

doi:10.1016/S1413-8670(10)70110-0

The Brazilian Journal of Infectious Diseases

ISSN: 1413-8670

The Brazilian Journal of Infectious Diseases is the official publication of the Brazilian Society of Infectious Diseases (SBI). It aims to publish relevant articles in the broadest sense on all aspects of microbiology, infectious diseases and immune response to infectious agents.
The BJID is a bimonthly publication and one of the most influential journals in its field in Brazil and Latin America with a high impact factor. Since its inception it has garnered a growing share of the publishing market.

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nsantos@micro.ufrj.br

Correspondence to: Departamento de Virologia, Instituto de Microbiologia, Paulo de Góes Universidade Federal do Rio de Janeiro, Cidade Universitária, CCS - Bl. I, Ilha do Fundão Rio de Janeiro - RJ, 21.941-590, Brazil Phone: 55 21 2560-8344 extension 165 Fax: 55 21 2560-8028.

Department of Virology, Instituto de Microbiologia, Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Brazil

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Abstract

Background

Diarrhea is a major cause of morbidity and mortality among HIV-infected patients worldwide. Objective: We sought to determine the frequency of viral gastrointestinal infections among Brazilian HIV-infected patients with diarrhea.

Methods

A collection of 90 fecal specimens from HIV-infected individuals with diarrhea, previously tested for the presence of bacteria and parasite was analyzed by polymerase chain reaction and sequence analysis for the presence of enteric viruses such as astrovirus, norovirus, rotavirus groups A, B and C, adenovirus, herpes simplex virus, Epstein-Barr virus, cytomegalovirus, and human bocavirus.

Results

Twenty patients (22.2%; n = 90) were infected with parasites (11 single infections and nine coinfected with virus). Enteropathogenic bacteria were not found. Virus infections were detected in 28.9% (26/90) of the specimens. Cytomegalovirus was the most common virus detected (24.4%; 22/90). Coinfections with viruses and/or parasite were observed in 10 (11.1%) samples.

Conclusion

Gastrointestinal virus infections were more frequent than parasitic or bacterial infections in this patient population.

Keywords:

HIV infection

gastrointestinal disorders

cytomegalovirus

human bocavirus

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