Elsevier

Virology

Volume 154, Issue 1, 15 October 1986, Pages 67-75
Virology

Dynamic and nonspecific dispersal of human T-cell leukemia/lymphoma virus type-I integration in cultured lymphoma cells

https://doi.org/10.1016/0042-6822(86)90430-7Get rights and content

Abstract

The progression of HTLV-I proviral integration over a 3-year period of in vitro culture was examined in two human lymphoma lines, Hut 102 and MJ. Using specific HTLV-I molecular clones and a Southern analysis at different cell passages, Hut 102 increased from 2 to 19 integrated proviral integrations while MJ increased to at least 25 different integrations by passage 43. During the progress of increased superinfection and novel integration in vitro some of the previous proviral integrations were lost from the cultures. The 19 integrations of late passage Hut 102 cells were shown to be dispersed to 19 different human chromosomes by analysis of 34 distinct rodent × Hut 102 somatic cell hybrids which segregated human chromosomes (and included proviral integrations) in different combinations. The two primary integrations in Hut 102 were located on human chromosomes 4 and 20, respectively. A similar pattern of nonspecific integration was observed in somatic cell hybrid analysis of the 25 proviral integrations of MJ. The dynamic infection-reintegration process in vitro revealed in these studies may confuse experimental verification of potential cis acting functions of HTLV-I in the as yet poorly understood mechanism of neoplastic transformation.

References (41)

  • W.A. Blattner et al.

    The human type-C retrovirus, HTLV, in blacks from the Caribbean region, and the relationship to adult T-cell leukemia/lymphoma

    Int. J. Cancer

    (1982)
  • M. Bobro et al.

    Differential staining of human and mouse chromosomes in interspecific cell hybrids

    Nature (London)

    (1974)
  • R.C. Gallo et al.

    Association of the human type C retrovirus with a subset of adult T-cell cancers

    Cancer Res.

    (1983)
  • F.D. Gillin et al.

    8-Azaguanine resistance in mammalian cells. I. Hypoxanthine-guanine phosphoribosyltransferase

    Genetics

    (1972)
  • W.C. Goh et al.

    Subcellular localization of the product of the long open reading frame of human T-cell leukemia virus type I

    Science

    (1985)
  • H. Harris et al.
  • W.S. Hayward et al.

    Activation of a cellular one gene by promoter insertion in ALV-induced lymphoid leukosis

    Nature (London)

    (1981)
  • V.S. Kalyanaraman et al.

    Immunological properties of a type C retrovirus isolated from cultured human T-lymphoma cells and comparison to other mammalian retroviruses

    J. Virol.

    (1981)
  • R.J. Klebe et al.

    Controlled production of proliferating somatic cell hybrids

    J. Cell Biol.

    (1970)
  • T.H. Lee et al.

    Antigens encoded by the 3′-terminal region of human T-cell leukemia virus: Evidence for a functional gene

    Science

    (1984)
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