Elsevier

Journal of Microbiological Methods

Volume 109, February 2015, Pages 157-159
Journal of Microbiological Methods

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Fast and reliable bacterial identification direct from positive blood culture using a new TFA sample preparation protocol and the Vitek® MS system

https://doi.org/10.1016/j.mimet.2014.12.009Get rights and content

Abstract

A simple and reliable protocol using 0.1% trifluoroacetic acid (TFA) was developed to identify bacteria directly from blood cultures on the Vitek® MS system. Results presented a correlation of 99% for Gram-negative bacteria at species level, and 86.3% and 82.3% of Gram-positive bacteria at the genus and species levels, respectively.

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Acknowledgment

We would like to thank Dr. Suzane Silbert (Tampa General Hospital, Tampa, FL, USA) for reviewing this manuscript.

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