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Vol. 21. Issue 4.
Pages 464-467 (July - August 2017)
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Vol. 21. Issue 4.
Pages 464-467 (July - August 2017)
Brief communication
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Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV and V isolated from healthy children attending public daycares in northeastern Brazil
Suzi P. de Carvalhoa,b, Jéssica B. de Almeidaa,b, Yasmin M.F.S. Andradeb, Lucas S.C. da Silvab, Arianne C. de Oliveirab, Flávia S. Nascimentob, Guilherme B. Camposc, Márcio V. Oliveirab, Jorge Timenetskyc, Lucas M. Marquesa,b,c,
Corresponding author

Corresponding author.
a Universidade Estadual de Santa Cruz, Departamento de Microbiologia, Ilhéus, BA, Brazil
b Universidade Federal da Bahia, Instituto Multidisciplinar em Saúde, Núcleo de Tecnologia em Saúde, Vitória da Conquista, BA, Brazil
c Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brazil
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Tables (2)
Table 1. Microbiological aspects and genetic characteristics of 11 MRSA isolates carriage from infants attending DCCs in Vitória da Conquista, Brazil.
Table 2. Biofilm production by MRSA and MSSA isolates recovered from nasal secretion of healthy children attending daycare centers in Vitória da Conquista, Bahia, Brazil.
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Nasal colonization with methicillin-resistant Staphylococcus aureus (MRSA) have increasingly been reported in healthy communities. This study aimed to assess the rate of S. aureus in general and MRSA in particular from nasal secretion of children in daycare centers in Vitória da Conquista, Brazil. The isolates were identified based on morphology, biochemical tests and by PCR. Detection of virulence genes, biofilm production, and susceptibility test by disk diffusion agar were performed. MRSA isolates were characterized by spa, SCCmec, and multilocus sequence typing (MLST). S. aureus were recovered from 70 (47.3%) of 148 children. Among the 11 MRSA strains (15.7%), two SCCmec types (IV and V) were detected. MLST identified four STs related to three clonal complexes (CC): 5, 45, and 398. Four spa types were found circulating in this setting. Resistance of S. aureus isolates to ampicillin, erythromycin, ciprofloxacin, clindamycin, and tetracycline was 80%, 32.8%, 7.1%, 7.1% and 4.3%, respectively. One isolate presented intermediate resistance to vancomycin detected by Etest methodology. All strains were biofilm producers. The virulence genes seb, sec, spa, and pvl were detected in some isolates. This study revealed a high rate of children carrying MRSA among healthy attendees in daycare centers in Vitória da Conquista, Brazil.

Antibiotic resistance
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Staphylococcus aureus may cause various infections with considerable morbidity and mortality in healthy and immunocompromised hosts.1 Methicillin-resistant Staphylococcus aureus (MRSA) is commonly associated with severe nosocomial infections (HA-MRSA); however, it has been detected in individuals without risk factors for infection, referred to as community-acquired MRSA (CA-MRSA).2 Methicillin resistance is carried on a staphylococcal cassette chromosome mec (SCCmec). HA-MRSA usually carry SCCmec types I, II, or III, whereas CA-MRSA strains commonly carry SCCmectypes IV and V.3

The molecular characterization is important, since it enables studying the relatedness of MRSA strains, their genetic diversity, and clonal distribution. Various molecular typing techniques have been developed, including the multilocus sequence type (MLST), as well as the SCCmec typing, spa typing.3–5

Nasal colonization of S. aureus is common in children, but the genetic findings suggested a causal relationship between carriers, chromosome cassettes of those harboring MRSA, and invasive staphylococcal disease.6 In addition, the incidence of pediatric infections due to CA-MRSA, including children with no identifiable risk factors, has increased worldwide.7 Daycare centers (DCCs) are reservoirs of S. aureus in general and MRSA in particular, and these children may spread these bacteria to the community and hospitals.6

In Brazil little is known about the distribution and characteristics of S. aureus and MRSA in children as nasal carriers, particularly in those attending DCCs. The aim of the present study was to assess the rate of S. aureus and MRSA nasal carriage in healthy children attending public DCCs in Vitória da Conquista – Bahia State (BA), Brazil, and to identify the resistance profile, genotypic characterization, and pathogenicity.

The study was conducted from October to December 2012, in four public DCCs in Vitória da Conquista, a city in Northeastern Brazil. One hundred and forty-eight samples of nasal swabs were obtained from healthy children ranging from one to six years attending DCCs. Children treated with antibiotics in the last 30 days were not included. The isolates obtained were incubated at 37°C for 48h; Gram-positive cocci colonies, positive for catalase and coagulase tests were selected as presumptive S. aureus and identified by PCR.

MRSA isolates and reduced vancomycin activity were screened for all S. aureus isolates by microdilution method following the recommendations of the CLSI guidelines.8 Strains of S. aureus ATCC 29213 and ATCC 43300 were used as controls. Vancomycin-resistant isolates were confirmed using the E-test® (bioMérieux's, Brazil) and the bacterial growth at ≥16μg/mL was indicative of resistance. Susceptibility to erythromycin, tetracycline, clindamycin, ciprofloxacin, and ampicillin were performed by disk diffusion.8 Inducible resistance to clindamycin was tested by ‘D test’.8 Multidrug resistance was considered when the strain was resistant to two or more antibiotics.

The genomic DNA of all strains were extracted using the Genomic DNA Purification Kit (Invitrogen, Carlsbad, CA, USA), and all isolates were characterized as SCCmec types (I–V),3 and for the presence of virulence genes, including sea, seb, sec, pvl, clfA, and spa by PCR.9 Moreover, MRSA isolates were subjected to MLST and SPA typing techniques, as described previously.4,5

Biofilm assays were performed in 96-well polystyrene microplates using trypticase soy broth (TSB/Difco) with 1% (w/v) glucose (TSB-1% Glc).10 The samples were compared with cultures of Streptococcus pyogenes ATCC75194 (non-biofilm producer sample). S. aureus isolates were classified as non-biofilm producers, weak producers, moderate producers, producers, and strong producers. Statistical analysis was performed by chi-square test and a p-value lower than 0.05 was considered statistically significant.

Out of 148 children analyzed, S. aureus was recovered from 70 (47.3%). Eleven (15.7%) isolates were MRSA, the majority (n=8; 72.7%) were SCCmec IVA type and three (27.2%) were SCCmec V type.

Resistance rates of S. aureus isolates to ampicillin, erythromycin, clindamycin, ciprofloxacin, and tetracycline were 80%, 32.8%, 7.1%, 7.1%, and 4.3%, respectively. One methicillin-susceptible S. aureus (MSSA) isolate showed reduced susceptibility to vancomycin by E-test and was confirmed with a MIC of 6μg/mL. There were no significant differences in resistance between MRSA and MSSA isolates. The rate of resistant isolates to more than two antibiotics was 47.1% (33/70), and only 15.7% (11/70) of the isolates were susceptible to the studied antibiotics. A total of 63.6% of MRSA isolates were multidrug resistant (Table 1).

Table 1.

Microbiological aspects and genetic characteristics of 11 MRSA isolates carriage from infants attending DCCs in Vitória da Conquista, Brazil.

Isolates  SCCmec typing  Resistance profilea  MIC (μg/mL)Phenotypeb  Virulence genes profiles  ST/CC  spa type 
(MRSA)      OXA  VAN         
C18  amp, ery  –  Moderate  spa  398/398  t242 
C48  IVA  amp, ery  Strong  spa  5/5  t242 
C54  IVA  amp  Strong  spa  5/5  t242 
C60  IVA  amp  Strong  seb, spa  2228/45  t004 
C77  IVA  amp, ery  –  Strong  spa  5/5  t242 
C80  IVA  –  –  Strong  spa  5/5  t002 
C83  amp, ery  Producer  seb, spa  398/398  t242 
C94  IVA  –  Strong  spa  5/5  t242 
C137  amp, cli, ery  –  Strong  spa  45/45  t371 
C138  IVA  amp, ery, tet  –  Strong  spa  5/5  t242 
C152  IVA  amp, ery, cip  –  Strong  spa  5/5  t242 

amp, ampicillin; ery, erythromycin; cip, ciprofloxacin; cli, clindamycin.


Moderate, moderate biofilm producer; producer, biofilm producer; strong, strong biofilm producer.

MLST performed on MRSA isolates identified four STs, related to three clonal complexes (CC): 5 (63.5%), 45 (18.2%), and 398 (18.2%). Among the four STs identified, the ST5 clone (63.6%, 7/11) was the most prevalent (Table 2). Protein A was charactered in isolates of four spa types. The t242 (72.7%, 8/11) was dominant among all isolates (Table 1).

Table 2.

Biofilm production by MRSA and MSSA isolates recovered from nasal secretion of healthy children attending daycare centers in Vitória da Conquista, Bahia, Brazil.

Samples  n  Non-producersWeak producersModerate producersProducersStrong producers
    n  n  n  n  n 
MRSA  11  9.1  9.1  81.8 
MSSA  59  6.7  11.8  12  20.3  36  61 
Total  70  5.7  11.4  13  18.5  45  64.2 

MRSA, methicillin-resistant Staphylococcus aureus; MSSA, methicillin-susceptible S. aureus.

Two MRSA isolates (2.8%) had the seb gene, and all had spa genes. Two strains of MSSA (2.8%) showed the sec gene, three (4.2%) were positive for pvl gene, and all had spa (Table 1). All S. aureus isolates produced biofilm (Table 2) and there was no statistical difference in biofilm production between MRSA and MSSA isolates (p>0.05).

The detection of S. aureus (47.3%) and MRSA (15.7%) in nasal swabs of the studied children demonstrated a higher rate of these stapphylococci than in studies conducted in other DCCs with rates ranging from 17% to 31.1% and 0.8 to 13.2%, respectively.7,11,12

Although the prevalence of MRSA among healthy children was high, no correlation was found between carriage of MRSA and antibiotic use. In addition, the close contact among DCC attendees has also been considered a risk factor for carring MRSA.6 A similar Brazilian study showed a MRSA prevalence of 1.2%12 confirming the variations between regions. The high incidence of MRSA in healthy children is the most relevant and worrying finding for the susceptible hosts and even for the healthy carrier children if immunosuppressed. This context involves at least the risk for coagulase-positive staphylococcal infections in elderly hosts, hospitalized individuals, neonates, and the community. Furthermore, most CA-MRSA reported worldwide have been shown to carry SCCmec types IV and V, as found in our results.11,13 The isolates detected by Ho et al. had SCCmec IV (46.4%) or V (53.6%),13 unlike Lamaro-Cardoso et al. who found SCCmec IIIA (57%) in most Brazilian children, which is widespread in hospitals in many countries worldwide, and only three MRSA strains showed SCCmec IV and one SCCmec type V genes.12 Therefore the distribution of the MRSA clone varies throughout the world.

The SCCmec IV has been strongly associated with strains causing MRSA infections in patients without risk factors in Brazil14 and elsewhere.15 Our findings point out the potential of this specific DCCs to be a reservoir of emerging MRSA genotypes and highlight the need to enhance surveillance of these bacteria and control their transmission.

The MLST and spa typing revealed a small diversity between CA-MRSA isolates. ST5 clones have emerged in hospital and community isolates, while ST45 is most often found in the community carrying SCCmecIV.16,17 The Clonal complexes – CC5 and CC45 – which represented 81.8% of CA-MRSA isolates in this study, are among the clonal groups known to be involved in global pandemic caused by MRSA. Meanwhile, invasive infection by CC398 rarely occurs.18

High rates of MRSA and MSSA isolates resistant to ampicillin (80%) and erythromycin (32.8%) were found, as well as low resistance rates to clindamycin, tetracycline, and ciprofloxacin. Our findings agree with previous studies in the literature, except for higher susceptibility to erythromycin elsewhere (14–20%).7 Thus, isolates of CA-MRSA had limited resistance to non-β-lactams. The majority of MRSA isolates (63.6%) detected herein were resistant to more than two antibiotics and this is a challenge in controlling these infections. One isolate (1.43%) with reduced susceptibility to vancomycin among MSSA strains is an important finding. Most cases of reduced vancomycin susceptibility in S. aureus (vancomycin-intermediate S. aureus, or VISA) reported in the literature are also methicillin-resistant. However, the development of VISA in MSSA isolates have been reported.19,20 Thus, reduced vancomycin susceptibility can occur in S. aureus irrespective of background methicillin susceptibility. There are no data in the literature of CA-MRSA strains susceptible to or with reduced susceptibility to vancomycin, so this fact is of particular concern in a healthy young population. Therefore, additional studies and continuous monitoring of S. aureus and MRSA are recommended.

Herein, all S. aureus isolates were able to produce biofilm, and the percentage of MRSA and MSSA strains producing biofilm was similar. There are no studies on biofilm production of S. aureus recovered from healthy individuals. There is no direct relationship of the methicillin resistance profile with greater or lesser ability to produce biofilm by the strains. The majority of biofilm producer isolates were classified as strong producers. This feature may act as an additional barrier to control an infection with antimicrobiotics.2

Protein A encoded by spa gene is a hallmark of S. aureus.15 Other virulence genes such as seb, sec, and pvl were detected in a few isolates. In a study of strains isolated from healthy students in Turkey, the sec gene was found to be the most frequent, followed by sea and none of the S. aureus isolates had pvl.21 The pvl gene has been shown to occurr less frequently in CA-MRSA isolates associated with asymptomatic nasal colonization, consistent with the results of the present study.22 The genes of staphylococcal enterotoxins have been implicated as important determinants of S. aureus virulence2; however, they have been poorly studied in healthy children in the community.

In conclusion, this study showed high detection rates of S. aureus and MRSA (7.4%) in healthy Brazilian day care children. Moreover, the characterized isolates with reduced sensitivity to vancomycin and biofilm production are of special concern. Thus, continuous surveillance of S. aureus and MRSA in Vitória da Conquista should be encouraged to gain a better understanding of the circulating staphylococci in order to establish better control of possible infections.

Conflicts of interest

The authors declare no conflicts of interest.


This study was supported by CAPES. We thank the DCCs, children and parents/guardians for allowing this research to be conducted, and for proofreading.

B. Shopsin, B. Mathema, J. Martinez, E. Ha, M.L. Campo, A. Fierman.
Prevalence of methicillin-resistant and methicillin-susceptible Staphylococcus aureus in the community.
J Infect Dis, 182 (2000), pp. 359-362
R.R. Watkins, M.Z. David, R.A. Salata.
Current concepts on the virulence mechanisms of methicillin-resistant Staphylococcus aureus.
J Med Microbiol, 61 (2012), pp. 1179-1193
K. Zhang, J.A. McClure, S. Elsayed, T. Louie, J.M. Conly.
Novel multiplex PCR assay for characterization and concomitant subtyping of Staphylococcal cassette chromosome mec Types I to V in methicillin-resistant Staphylococcus aureus.
J Clin Microbiol, 43 (2005), pp. 5026-5033
M.C. Enright, N.P. Day, C.E. Davies, S.J. Peacock, B.G. Spratt.
Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus.
J Clin Microbiol, 38 (2000), pp. 1008-1015
D. Harmsen, H. Claus, W. Witte, et al.
Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management.
J Clin Microbiol, 41 (2003), pp. 5442-5448
M.B. Miller, D.J. Weber, J.S. Goodrich, et al.
Prevalence and risk factor analysis for methicillin-resistant Staphylococcus aureus nasal colonization in children attending childcare centers.
J Clin Microbiol, 49 (2011), pp. 1041-1047
D.A. Tavares, R. Sa-Leao, M. Miragaia, H. de Lencastre.
Large screening of CA-MRSA among Staphylococcus aureus colonizing healthy young children living in two areas (urban and rural) of Portugal.
BMC Infect Dis, 10 (2010), pp. 110
Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Second Informational Supplement. CLSI document M100-S22.
Clinical and Laboratory Standards Institute, (2012),
P.C. Proietti, G. Coppola, A. Bietta, M.L. Marenzoni, D.R. Passamonti Hyatt.
Characterization of genes encoding virulence determinants and toxins in Staphylococcus aureus from bovine milk in Central Italy.
J Vet Med Sci, 72 (2010), pp. 1443-1448
J.E. Cassat, C.Y. Lee, M.S. Smeltzer.
Investigation of biofilm formation in clinical isolates of Staphylococcus aureus.
Methods Mol Bio, 391 (2007), pp. 127-144
W.T. Lo, W.J. Lin, M.H. Tseng, J.J. Lu, M.L. Chu, C.C. Wang.
Nasal carriage of a single clone of community-acquired methicillin resistant Staphylococcus aureus among kindergarten attendees in northern Taiwan.
BMC Infect Dis, 7 (2007), pp. 51
J. Lamaro-Cardoso, H. Lencastre, A. Kipnis, et al.
Molecular epidemiology and risk factors for nasal carriage of Staphylococcus aureus and methicillin-resistant S. aureus in infants attending daycare centers in Brazil.
J Clin Microbiol, 47 (2009), pp. 3991-3997
P.L. Ho, S.S. Chiu, M.Y. Chan, et al.
Molecular epidemiology and nasal carriage of Staphylococcus aureus and methicillin-resistant S. aureus among young children attending daycare centers and kindergartens in Hong Kong.
J Infect, 64 (2012), pp. 500-506
L.C. Gelatti, R.R. Bonamigo, F.M. Inoue, et al.
Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil.
Rev Soc Bras Med Trop, 46 (2013), pp. 34-38
M.V. David, R.S. Daum.
Community-associated methicillin-resistant Staphylococcus aureus: epidemiology and clinical consequences of an emerging epidemic.
Clin Microbiol Rev, 23 (2010), pp. 616-687
M.A. de Sousa, T. Conceicao, C. Simas, H. De Lencastre.
Comparison of genetic backgrounds of methicillin-resistant and-susceptible Staphylococcus aureus isolates from Portuguese hospitals and the community.
J Clin Microbiol, 43 (2005), pp. 5150-5157
X. Xie, Y. Bao, N. Ouyang, et al.
Molecular epidemiology and characteristic of virulence gene of community-acquired and hospital-acquired methicillin-resistant Staphylococcus aureus isolates in Sun Yat-sen Memorial hospital, Guangzhou, Southern China.
BMC Infect Dis, 16 (2016), pp. 339
K. Krziwanek, S. Metz-Gercek, H. Mittermayer.
Methicillin-resistant Staphylococcus aureus ST398 from human patients, upper Austria.
Emerg Infect Dis, 15 (2009), pp. 766-769
S.K. Pillai, C. Wennersten, L. Venkataraman, G.M. Eliopoulos, R.C. Moellering, A.W. Karchmer.
Development of reduced vancomycin susceptibility in methicillin-susceptible Staphylococcus aureus.
Clin Infect Dis, 49 (2009), pp. 1169-1174
J. Hu, X.X. Ma, Y. Tian, L. Pang, L.Z. Cui, H. Shang.
Reduced vancomycin susceptibility found in methicillin-resistant and methicillin-sensitive Staphylococcus aureus clinical isolates in Northeast China.
H.T. Dağı, D. Fındık, G. Demirel, U. Arslan.
Detection of methicillin resistance and various virulence factors in Staphylococcus aureus strains isolated from nasal carriers.
Balkan Med J, 32 (2015), pp. 171-175
S. Boyle-Vavra, R.S. Daum.
Community-acquired methicillin-resistant Staphylococcus aureus: the role of Panton–Valentine leukocidin.
Laby Invest, 87 (2007), pp. 3-9
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